- Maltopentaose 생산 균의 분리 및 생산 조건 연구
- ㆍ 저자명
- 김영민,서은성,김도만,김도원,이진하
- ㆍ 간행물명
- 한국생물공학회지
- ㆍ 권/호정보
- 2001년|16권 3호|pp.246-252 (7 pages)
- ㆍ 발행정보
- 한국생물공학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
We isolated a bacterium that produces an extracellular maltopentaose(G5)-forming amylase from amylose and soluble starch. The bacterium was identified and assigned as a Bacillus sp. AIR-5. The amylase did not hydrolyze maltose, maltotriose, maltotetraose or maltopentaose. Optimum medium composition for maltopentaose production in flask culture was 2%(w/v) soluble starch, 0.4%(w/v) tryptone, 0.5%(w/v) NaCl, 0.5%(w/v) K$_2$HPO$_4$, and 3 mM CaCl$_2$at pH 8.0, 28$^{circ}C$. The highest yield for maltopentaose production in this condition was 6.45 g/L and was 32.55% of theoretical yield.