Film cooling performance from two rows of holes with opposite orientation angles is evaluated in terms of heat flux ratio. The film cooling hole has a fixed inclination angle of 35°and orientation angle of 45°for the downstream row and -45°for the upstream row. Four film cooling hole arrangements including inline and staggered configurations are investigated. The blowing ratio studied was 1.0. Boundary layer temperature distributions are measured to investigate injectant behaviors and mixing characteristics. Detailed distributions of the adiabatic film cooling effectiveness and the heat transfer coefficient are measured using TLC(Thermochromic Liquid Crystal). For the inline configuration, there forms a downwash flow at the downstream hole exit to make the injectant well attach to the wall, which gives high adiabatic film cooling effectiveness and heat transfer coefficient. The evaluation of heat flux ratio shows that the inline configuration gives better film cooling performance with the help of the downwash flow at the downstream hole exits.