The in-cylinder flow field of gasoline engine comprises unsteady compressible turbulent flows caused by the intake port, combustion chamber geometry. Thus, the quantitative analysis of the in-cylinder flow characteristics plays an important role in the improvement of engine performances and the reduction of exhaust emission. In order to obtain the quantitative analysis of the in-cylinder gas flows for a gasoline engine, the single-frame particle tracking velocimetry was developed, which is designed to measure 2-dimensional gas flow field. In this paper, influences of the swirl and tumble intensifying valves on the in-cylinder flow characteristics under the various intake flow conditions were investigated by using this PTV method. Based on the results of experiment, the generation process of swirl and tumble flow in a cylinder during intake stroke was clarified. Its effect on the tumble ratio at the end of compression stroke was also investigated.