In this paper, we propose a novel robust fault isolation filter design method using the left eigenstructure assignment scheme proposed by the authors. The proposed method guarantees that the ${gamma}$ simultaneous faults can be isolated when the number of available outpur measurements is ${gamma}$. Moreover, if there exist redundant output measurements, the eigenvaluses of te filter system can be assigned to the desired position or the filter can be designed robustly to, the system parameter variation. Liu & Si developed a filter design method which has the same purpose, fault isolation. However their method cannot use the redundant freedom of the output matrix C. The proposed filter can use the redundant freedom of the matrix C effectively. Beside this in this paper, an eigenstructure assignment methodology that satisfies the required fault isolation conditions is also proposed. The proposed fault isolation filter was applied for isolating the simultaneous faults to a VTOL aircraft in order to verify the fault isolation performance.