The research described in this paper was aimed toward improving the understanding of the interaction of tire inflation pressure and the soil-tire interface stresses. A three-directional stress transducer was developed to measure stress distribution on undertread for a tractor tire. The transducer can directly measure three-directional stresses (normal stress, tangental stress and lateral stress and lateral stress) simultaneously and has both strong structure and high sensitivity, which is not changed by the abrasion of the detecting plate. Measurements of soil-undertread interface stresses were made at tire center on undertread on a 12.4-R24 radial tractor tire opeated at three combinations of a dynamic load (11.8kN) and three inflation pressures (59kPa, 108kPa and 157kPa). These measurements showed that as inflation pressure increased, the soil-undertread interface stresses increased. The results of three stresses comparisons were shown that the peak normal stresses were considerably higher than the tangential peak stresses and the peak lateral stresses.