This study deals with the effect on non-martensitic layer on the fatigue strength in carburized gear. The test gears are carburized, then treated by the combination of chemical polishing and electro-polishing. Carburization treatment is used widely on parts of power transmission system like surface hardened layer to improve fatigue strength. Carburized gears are observed using a scanning electron microscope(SEM) to determine the characteristics of crack initiation mechanism in the surface layer. The constant street amplitude fatigue test is performed by using and electro-hydraulic servo-controlled pulsating tester. The S-N curves are obtained and illustrated. The effect of non-martensitic layer on the fatigue strength is clarified.