In the present work, a linear static analysis is presented for thin-walled prismatic box-beams made of generally anisotropic materials. A mixed beam theory has been used to model and carry out the analysis. Several different constitutive assumptions for the shell-wall of the beam section are assessed into the beam formulation. Simple layup cases of box-beams representing bending-torsion or extension-torsion coupled configuration have been considered and tested to clearly show the effects of elastic couplings of the beam. A detailed finite element structural analysis using the MSC/NASTRAN has been carried out to validate the current analytical results. Numerical results show that appropriate assumptions for the constitutive relations are important and crucial for the accurate prediction of beam stiffness constants and also thor the beam behavior.