This study presents the estimation of the entrainment velocity of sediment particles from the scour hole. Sediment particles are entrained from the scour hole downstream of hydraulic structures by the turbulent vortices. Mathematical form of the entrainment velocity of sediment particles from the scour hole was obtained using the impulse-momentum equation with given value of the vertical component of turbulent velocity of the line vortex. Also, its probability density distribution was obtained with the results that the probability density distribution of the vertical turbulent velocity followed the normal distribution. Experimental results of the entrainment velocity of sediment particles showed relatively good agreements with theoretical ones.