In this paper, a coaxial sample holder is proposed with its de-embedding and parameter extraction procedure. The S-parameters were measured up to 1 GHz using network analyzer, HP8753D, and N-type connector together with the de-embedding of N-type connector. The proposed de-embedding procedure is performed to extract electrical parameters of blood glucose, which gives the permittivity of blood glucose. We also analyzed the error of extracted parameters, which are caused by instrument error and geometrical error. Using these error analyses, we reduced the error factors of extracted parameters. We extracted electrical parameters of glucose samples through these all extraction procedure and confirmed the possibility of glucose diagnosis system based on microwave system