Activated carbon particles of 1.274 mm diameter and sand particles of 0.455 mm diameter were employed as the support of the biofilm formed in fluidized bed biofilm reactors(FBBRs) for the wastewater denitrification. Ethanol was used as the electron donor in the anoxic respiration. The steady-state biofilm thickness increased as the nitrate loading rate increased, and the activated carbon particles induced thicker biofilm than the sand particles. The FBBRs with sand support showed higher efficiency and rate of the nitrate removal than those with activated carbon support, and exhibited the biomass concentration of 37 kg/㎥ and the nitrate removal rate of 21 kg N/㎥d.