본 연구는 천연기념물로 지정되어 있는 제주마의 유전자원 보존과 증식을 위한 인공수정 기술의 실용화를 위한 기초자료를 제공하고자 제주도 축산진흥원에서 사육되고 있는 제주마 종마 4두를 공시하여 Missouri style 인공질을 이용 정액을 채취하여 사정 특성 및 정액의 일반 성상을 조사하였다. 제주마는 교미를 위하여 발정 중인 암말에 사정당 2.3$pm$1.8회 승가를 하며 사정시간은 27.0$pm$12.5초였다. 정액의 일반성상을 조사한 결과 총 정액량은 47.8$pm$26.7ml, gel-free 양은 42.7$pm$27.4ml, 정자농도는 270.7$pm$112.9$ imes$$10^{6}$ml로 사정당 총 정자수는 7.6$pm$3.9$ imes$$10^{9}$ ml였다. 또한 원정액중 생존 정자의 비율은 75.0$pm$18.2%로 사정당 생존 정자수는 6.1$pm$3.4$ imes$$10^{9}$ ml였으며 gel-free 정액의 pH는 7.3$pm$0.2이었다. 정자의 기형율은 평균 31.5%로서 부위별로는 미부가 15.0$pm$15.0%, 두부가 9.5$pm$11.7% 그리고 경부가 7.0$pm$4.0%였다.
The Jeju horse has been raised for centuries in Jeju island. Recently, as the number of this indigenous horse has been dropped dramatically, this breed became Natural Monument #347 to conserve and multiply this endangered breed. To provide the basic information for AI, sexual activity and semen characteristics in Jeju horse were investigated. Jeju horse semen was collected using Missouri style artificial vagina from fertile stallion.\`she number of mount per ejaculation was 2..3$pm$1.8, and the ejaculation time was 27.0$pm$12.5 seconds. The total volume and gel-free volume of semen was 47.8$pm$26.7 ml and 42.7$pm$27.4ml, respectively, and the concentration of sperm and the total number of spermatozoa per ejaculation was 200.7$pm$112.9$ imes$10$^{6}$ ml and 7.6$pm$3.9$ imes$10$^{9}$ ml, respectively. The percentage of motile sperm and the number of live spermatozoa per ejaculation was 75.0$pm$18.2% and 6.1$pm$3.4$ imes$10$^{9}$ ml, respectively, and the pH of gel-free semen was 7.3$pm$0.2. The total percentage of abnormal sperm was 31.5%, and the percentage of sperm with abnormal head, midpiece and tail was 9.5$pm$11.7%, 7.0$pm$4.0% and 15.0$pm$15.0%, respectively.
