In this study, ground reaction force(GRF) and energy consumption of fixed. single-axis and multi-axis Prosthetic ankle assemblies were investigated to show the biomechanical evaluation for trans-tibial amputees. In the experiments. two male and two female trans-tibial amputees were tested with fixed, sin91e-axis and multi-axis Prosthetic ankle assembly. A three-dimensional gait analysis was carried out to derive the ratio of GRF to weight as the percentage of total stance Phase for nine Points Energy consumption of each Prosthetic ankle assembly was measured while subjects walked at 2km/h. 3km/h and the most comfortable walking speed on the treadmill The results showed that multi-axis ankle was superior to the other two ankle assemblies for the characteristic of forwarding and breaking forces. Fixed ankle was relatively superior to the other two ankle assemblies for gait balancing and movement of the center fur mass Compared to the other ankle assembly. sing1e-axis type showed lower energy consumption over 2.3km/h walking speed .