Micro-cuttings (shoots with two small leaves) of cultivar M9 apple were cultured in-vitro for 40 d under $CO_2$-enriched and non-enriched (i.e., ambient air) conditions, and at a PPF of 40 or 100 $mu$mol $m^{-2}$ $s^{-1}$ . Afterward, shoot length, number of leaves, leaf area, chlorophyll content, shoot and root fresh weights, and % survival were recorded. Those plant-lets grown under C $O^{2-}$ and PPF-enriched treatments were healthy and vigorous, and showed higher values for their growth parameters. In contrast, those grown without supplemental $CO_2$ or PPF often showed hyperhydricity, We also demonstrated that $CO_2$ enrichment and a relatively high PPF during in-vitro culture promoted normal photosynthesis and growth after ex-vitro transplantation.