The present study is to assess the applicability of the best-estimate thermal-hydraulic code, MARS 1.4, for the analysis of thermal-hydraulic behavior in PWRs during natural circulation conditions. The code simulates a natural circulation test, BETHSY test 4. la, which was conducted on the integral test facility of BETHSY. The test represented the cooling states of the primary cooling system under single-phase natural circulation, two-phase natural circulation and the reflux condensation mode with conditions corresponding to the residual power, 2% of the rated core power value and 6.8 MPa at the secondary system. Based on MARS 1.4 calculations, the major thermal-hydraulic behaviors during natural circulation are evaluated and the differences between the experimental data and calculated results are identified. The calculated results show generally good behavior with regard to the experimental results; the region of single-phase natural circulation is 100-92% of the initial mass inventory, two-phase natural circulation is 84-63 %, and the reflux condensation mode occurred below 58 %. U-tubes empty and the core uncovery are obtained at 39 % and 34 % of the initial mass inventory, respectively.