This study was to determine the effect of garment closures and layering systems on insulation, using a thermal movable manikin in cold weather conditions. The insulation values of ensembles with opened and closed features were measured, and those of four different layered clothing ensembles were tested while standing and while walking. Our research indicated that when there was an opening involved in design the system, insulation decreased; even a zip-out lining in the armpit affected little. If a light weight jacket and pants are put on over a fleece shirt and pants instead polyester underwear, the amount of insulation increase was 0.43 clo.