Low back disorders (LBDs) are one of the most common and costly work-related musculoskeletal disorders. One of the major possible risk factors of LBDs is to work with static and awkward trunk postures, especially in a complex trunk posture involving flexion, twisting and lateral bending simultaneously. This study is to examine the effect of complex trunk postures on the postural stresses using a psychophysical method. Twelve healthy male students participated in an experiment, in which 29 different trunk postures were evaluated using the magnitude estimation method. The results showed that subjective discomfort significantly increased as the levels of trunk flexion, lateral bending and rotation increased. Significant interaction effects were found between rotation and lateral bending or flexion when the severe lateral bending or rotation were assumed, indicating that simultaneous occurrence of trunk flexion, lateral bending and rotation increases discomfort ratings synergistically. A postural workload evaluation scheme of trunk postures was proposed based on the angular deviation levels from the neutral position. Each trunk posture was assigned numerical stress index depending upon its discomfort rating, which was defined as the ratio of discomfort of a posture to that of its neutral posture. Four qualitative action categories for the stress index were also provided in order to enable practitioners to apply corrective actions appropriately. The proposed scheme is expected to be applied to several field areas for evaluating trunk postural stresses.