The seasonal variation of two triterpene glycosides, madecassoside and asisaticoside, in the aerial parts of Centella asiatica from May through October was investigated. In leaves, the highest level of triterpene glycosides content was about 108.1 mg/g in September. In petiols, the content was as low as 19.02 mg/g. According to the experimental results obtained, we found that Centella asiatica leaf can accumulate the triterpene glycosides and September is the optimal season for the collection.