The fatigue process of the triceps surae was evaluated during a standing heel-rise test, comparising of pre-fatigue and post-fatigue. Sixteen healthy subjects with a mean age of 22.88 years participated. The heel-rise test was performed until exhaustion, and electromyographic activity expressed as mean value and root mean square of the gastrocnemius and soleus muscles were calculated. The average number of heel-rise performed was 63.69$pm$26.10. The result of this present study indicate that the EMG signal is sensitive to the different muscle fatigue. Mean value and RMS decreased significantly in after fatigue. Significant difference were found between the pre-fatigue and post-fatigue in the total subjects. But there were no significant changes in mean and RMS between the male and female during standing heel-rise test. The present results, showing decreased EMG signal in the triceps surae, could be used as reference when testing the fatigue process in different clinical conditions. Recommendations for standardization of a heel-rise test are given.