A method for simulating flow of extremely low Reynolds number in a vortex-in-cell (VIC) was studied. The viscous diffusion was represented by the random walk method. The validity of this method was proven by applying it to the flows passing over a fence placed vertically at an extremely low Reynolds number. The effects of parameters of the VIC method on the random walk method, such as the number of point vortices, the mesh density and the time increment, were investigated by numerical analysis using a one-dimensional diffusion equation. Changes in the relative error of vorticity depending on those parameters were clarified.