In a previous experiment, pectin was the binding material between the cotton fibers and the seed coat. Based on the results, several enzymes were chosen to treat cotton fabrics in order to investigate the effect of enzymatic treatment on seed coat fragment removal from the cotton fabrics. This experiment indicated that the enzymes used did not remove the seed coat fragments from the fabric by themselves. However, seed coat fragment removal and fabric whiteness improved with specific enzymatic pre-treatments followed by bleaching compared to bleaching only. Among the enzymes, one of the amylase showed the must enhancement in seed coat fragment removal. The effect of the artylase pre-treatment on the fabric whiteness and the seed coat fragment removal was as large as conventional alkaline scouring.