It is known that house dust mites which settle on sofa, carpet, and dust in the house ignite asthma and allergic rhinitis. Dermatophagoides farinae and Dermatophagoides pteronyssinus distribute widely and densely in Korea. In this study we carried out to identify that shed hair of dogs which was applied topically with fipronil ($Frontline^{circledR}$) kill house dust mites. We co-cultured house dust mites with fipronil-applied hair of dog during 12 hours, and then compared the death rate of mites here with that cultured without hair. The larger amount of hair, the more mites were killed. The death rate of mites was higher than control significantly until 2 weeks after $Frontline^{circledR}$ application. Hair which had been shed at 3 days after $Frontline^{circledR}$ application always killed the smaller number of mites than not shed. The present study suggested that the use of $Frontline^{circledR}$ to our pets control house dust mites effectively.