This study was designed to investigate the anti-tumor effects of fresh carrot juice, methanol-extracts, and $eta$-carotene on the human lung cancer cell line NCI-H1299. The anti-tumor effect was evaluated by the MTT assay in vitro. The anti-tumor effect of fresh carrot juice against NCI-H1299 lasted up to 96 hours after exposure; the viability rate of lung cancer cells decreased below 50% after 48 hours, and further after 72 hours. The strongest propagation inhibition effect of fresh carrot juice was shown at the concentration of 2000 $mu extrm{g}$/$mell$ after 72 hours and the viability rates was 45.98% even at the concentration of 25 $mu extrm{g}$/$mell$. The value of $IC_{50}$/ was 23.1$mu extrm{g}$/$mell$ when the elapsed time was 72 hours. The viability rate of methanol-extract was 52.4% under the concentration of 2000 $mu extrm{g}$/$mell$ and the elapsed time of 72 hours. Under the concentration of 1000 $mu extrm{g}$/$mell$ and the elapsed time of 48 hours, $eta$ -carotene decreased the viability rate to 29.99%. The $IC_{50}$/ value of $eta$-carotene was 691.2$mu extrm{g}$/$mell$ after 72 hours. According to the above results, the anti-tumor effect arose in NCI-H1299 when the concentration of the fresh carrot juice or the $eta$-carotene was more than 25 $mu extrm{g}$/$mell$ or 1000 $mu extrm{g}$/$mell$, respectively. On the other hand, the methanol-extracts showed a weak anti-tumor effect even at a concentration as high as 2000 $mu extrm{g}$/$mell$.