In this Paper, It is analysed 5 GHz frequency characteristics that carry out interference experimental under the indoor environments for understanding propagation interference between existing 5 GHz band wireless communication system and 5 GHz band wireless LAN system. The interference signal which was generated by signal generator injected to 5 GHz Wireless LAN communication system measured interference characteristics and This radio propagation main interference factors were classified received power level separation by interference source frequency and interference source frequency pulse period & width separation. It is measured transmission rate according to variable data and packet size for conforming the quality of 5 GHz band wireless LAN system. We measured the packet data loss rate and power level of received signal and analyzed its frequency characteristics effected by interference signal.