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Monitoring Expression of bphC Gene from Ralstonia eutropha H85O Induced by Plant Terpenes in Soil
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  • Monitoring Expression of bphC Gene from Ralstonia eutropha H85O Induced by Plant Terpenes in Soil
  • Monitoring Expression of bphC Gene from Ralstonia eutropha H85O Induced by Plant Terpenes in Soil
저자명
Jung. Kyung-Ja,Kim. Byung-Hyuk,Kim. Eungbin,So. Jae-Seong,Koh. Sung-Cheol
간행물명
The journal of microbiology
권/호정보
2002년|40권 4호|pp.340-343 (4 pages)
발행정보
한국미생물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A PCB degrader, Ralstonia eutropha H850 was shown to induce bphC gene encoding 2,3-dihydroxy-biphenyl-1,2-dioxygenase in a carvone-amended pure culture in our previous study (Park et al.,1999). The present study was carried out to examine how plant terpenes, as natural substrates, would cause an expression of a PCB degradative gene in soil that was amended with terpenes. The population of Ralstonia eutropha H850 was maintained at least around 10$^$8/ (CFU/g fresh soil) in the soil amended with carvone or limonene in the presence of succinate as a growth substrate at 50 th day. The gene expression was monitored by RT-PCR using total RNA directly extracted from each soil and bphC gene primers. The bphC gene expression of the seeded strain H850 was observed in the soil amended with biphenyl (4 days) but not with succinate, carvone and limonene. These results indicate that terpenes widely distributed in nature could be a potential inducing substrate for effective PCB biodegration in the soil but their bioavailability and specific induction behavior should be taken into account before PCB bioremediation implementation.