A new and two known lupane-triterpene glycosides were isolated from the hot MeOH fraction of the leaves of Acanthopanax gracilistylus W. W. Smith. Based on the physical properties and spectroscopic data, their chemical structures were determined as acankoreoside A (1), acankoreoside D (2), and $3{alpha}-hydroxy-lup-23-al-20(29)-en-28-oic$ acid $28-O-{alpha}-L-rhamnopyranosyl-(1{ ightarrow}4)-{$beta}-D-glucopyranosyl-(1{ ightarrow}6)-{eta}-D-glucopyranosyl$ ester (3), respectively. To our best knowledge, compand 3 appears to be novel, which was named as wujiapioside A.