본 연구의 목적은 납 중독된 쥐의 신장에서 키토산 효과를 연구하고자 하였다. 납(30 mg/kg)은 1주 2회 복강투여하고, 0.1% 키토산(1 mg/ml) 수용액은 음용수를 통해 자유자재로 공급하였다. 실험 후, 4주, 8주째에 각각의 신장을 적출해서 투과전자 현미경으로 관찰하였다. 실험군의 경우 Group 1은 납 중독 후 키토산용액을 처치하지 않은 군, Group 2는 납 중독 후 키토산용액을 처치한 군으로 각 군당 10마리의 쥐를 사용하였다. 실험 결과는 다음과 같다. 납 단독투여군의 경우 세뇨관 세포에서 미세융모의 소실이 나타났으며 용해소체가 증가되었다. 사립체 및 과립형질내세망 수조 내강의 팽대와 과립형질내세망으로부터 리보솜의 탈락이 관찰되었다. 납-키토산 투여군의 경우 세뇨관 세포에서 미세융모의 변화는 없었으며 용해소체의 수가 감소되었다. 기다란 모양의 사립체와 리보솜이 부착된 과립형질내세망이 정상적인 상태로 관찰되었다. 결론적으로, 키토산이 쥐의 신장에 미치는 납의 독성을 감소시키는데 효과가 있는 것으로 사료된다.
The purpose of this study was to evaluate the effect the lead toxicity of chitosan on the rats. A healthy male of Wistar rat was used for experiment. The lead acetate (30 mg/kg, Pb) was injected two times per a week into the abdomen. 0.1% chitosan solution (1 mg/ml) was supplied by feeding ad libitum. After 4 or 8 weeks, we was observed kidney by electron microscope. The experimental groups were divided into two groups. Group 1 was control group which was not treated with chitosan solution after contaminatation of lead acetate. Group 2 was experimental group which was treated with chitosan solution after contamination of lead acetate. All groups were used to 10 rats. The results were as follow: On the proximal convoluted tubules of the group with only Pb, the number of microvillis were decreased and the number of lysosomes were increased on the cytoplasm. The mitochondria and rough endoplasmic reticulum were extended and ribosomes dropped from the rough endoplasmic reticulum. On the proximal convoluted tubules of the group with Pb-chitosan, the shapes and number of microvilli were not changed and the number of lysosomes were decreased. The shapes of mitochondria and endoplasmic reticulum observed not changes. We concluded that chitosan has effect on reducing toxicity of lead in the rat kidney.
