Protective clothing for firefighters typically consists of a flame resistant outer shell and inner layers. The inner layers are generally composed of a moisture barrier and a thermal barrier. On performing the task in fire place the heat and perspiration generated from the body become trapped inside the protective clothing. Those heat and moisture result into heat-stress and physical fatigue of fire fighter, which hinder the work. Therefore, the system of clothing designs and material layers must be chosen carefully to balance protection and comfort. 3 kinds of protective clothing of 3 layer structure were used in the experiment of physiological comfort. From the comparison of wear trials with the 3 kinds of layers in firefighters clothing, it indicates that the moisture dissipation of A+B2+C was highest, following A+BI+C andA+B3+C. And the heat dissipation of A+BI+C and A+B2+C were better than A+B3+C. In the protective clothing with A+B3+C, heat and perspiration generated through exercise remained in clothing system long and caused discomfort.