쑥갓의 지상부를 MeOH로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. EtOAc 분획으로부터 silica gel column chromatography를 반복하여 3종의 sterol을 분리하였다. 각각의 화학구조는, NMR및 MS등의 스펙트럼을 해석하여, $stigmast-5-en-3{eta}-ol;(1,;{eta}-sitosterol),;stigmast-4-en-6{eta}-ol-3-one$ (2), $stigmast-4-en-6{alpha}-3-one$ (3)으로 동정하였다. 화합물 2와 3은 육상식물에서는 처음으로 분리되었다.
The aerial parts of tile Garland (Chrysanthemum comnarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. EtOAc fractions gave three steroid compounds through application of silica gel column chromatographies. The chemical structures of the steroids were determined by the interpretation of several spectral data, including NMR and MS as $stigmast-5-en-3{eta}-ol;(1,;{eta}-sitosterol),;stigmast-4-en-6{eta}-ol-3-one$ (2), $stigmast-4-en-6{alpha}-ol-3-one$ (3). Compounds 2 and 3 have been so far reported only in the aquatic plants, were isolated for the first time from the land plants.
