Objective : The purpose of this study was to investigate the effects of Bee Venom on the lipopolysaccharide(LPS), sodium nitroprusside(SNP), hydrogen peroxide$(H_2O_2)$-induced expression inducilble nitric oxide synthetase(iNOS), tumor necrosis factor-${alpha}$(TNF-${alpha}$) and nuclear factor kappa B(NF-kB) in RAW 264.7 cells, a murine macrophage cell line. Method : The expressions of expression iNOS and TNF-${alpha}$ were determined by western blotting with corresponding antibodies. The expressions of expression NF-kB was assayed by EMSA method. Results : 1. The 0.5, 1 and $5{mu}g/mg$ of bee venom on LPS-induced expression of iNOS, the $5{mu}g/mg$ of bee venom on SNP-induced expression of iNOS and the $1{mu}g/mg$ of bee venom on $H_2O_2$-induced expression of iNOS compared with control were inhibited significantly. 2. The 0.5, 1 and $5{mu}g/mg$ of bee venom inhibited significantly LPS and $H_2O_2$-induced expression of TNF-${alpha}$ compared with control, respectively. The $0.5{mu}g/mg$ of bee venom increased significantly SNP-induced expression of TNF-${alpha}$ compared with control. 3. The $5{mu}g/mg$ of bee venom on LPS-induced expression of NF-kB, the $0.5{mu}g/mg$ of bee venom on SNP-induced expression of NF-kB and the 0.5, $5{mu}g/mg$ of bee venom on $H_2O_2$-induced expression of NF-kB were inhibited significantly compared with control, respectively.