This paper presents the algorithms and Matlab Toolbox for co-simulation of distributed real-time control system based on OSEK-OS and CAN protocol. This toolbox enables the developers to analyze the timing uncertainty, which is caused by resource sharing including shared memories and networks, and to take the timing uncertainty into consideration in the early design phase. Furthermore, this toolbox helps the developers to model the behaviors of a control system by providing graphical user interface for objects of OSEK-OS and CAN. To prove the feasibility of this toolbox, a vehicle body network system is modeled with this toolbox, and the timing uncertainties are analyzed.