In this study, his technique was applied to a GDI spray in order to investigate the mixture distribution. In addition, the homogeneity degree and diffusion effect according to ambient temperature in the high pressure chamber were analyzed by using an entropy analysis method. From this experiment, we could find that entropy analysis is very effective method for the analysis of mixture formation, and the entropy values increase with the progress of uniformity in diffusion Process. we tried to provide the fundamental data for parameter which effects on the spray macroscopic characteristics with mixture ratio of diesel and gasoline. In addition, the mixture formation was analyzed by using entropy analysis. The entropy analysis is based on the concept of statistical entropy, and it identifies the degree of homogeneity in the fuel concentration. From the entropy analysis results we could find that the direct diffusion phenomena is a dominant factor in the formation of a homogeneous mixture at downstream of GDI spray especially in vaporizing conditions. As to increasing ambient temperature and increasing gasoline rate, the entropy intensity using the statistic thermodynamics method is increased because evaporation rate is higher gasoline than diesel.