The effect of pasting agent on the defibrillation of Tencel fabric was investigated. It was evaluated by the weigth loss of fabric when the fabric was treated with cellulase containing various kinds of pasting agents. The surface appearance of Tencel fabric was checked by SEM. Under the treatment condition without pasting agent, the weight loss of fabric was high at pH 5.0 and $60^circ{C}$. This means that the cellulase activity was high at this condition. By increasing the concentration of carboxymethyl cellulose(CMC), the weight loss of fabric was decreased monotonously. This tendency was not appeared in other pasting agents. CMC is synthesized by the reaction of chloroacetic acid and cellulose. The glucose units may be remained after the reaction. So, it was considered that the degradation of glucose unit in Tencel was decreased, because cellulase had to attack both Tencel and CMC.