The effect of 4 kinds of alcohols was investigated on the production of bacterial cellulose (BC) by Gluconacetobacter hansenii PJK. The addition of alcohols and acetic acid to medium caused the pellets of bacterial cellulose to aggregate into a lump, which could be easily separated from the culture medium. The growth rate of cells and the production yield of BC increased in the medium containing ethanol. Other alcohols in the medium decreased cell growth and the cellulose production rate, because of their toxic effects. The addition of ethanol depressed the conversion of a $ extrm{Cel}^{+}$ cell to a $ extrm{Cel}^{-}$ mutant in shaking culture. Cells subcultured three in a medium containing ethanol produced BC without any loss of BC production yield.