A newly developed contact angle measurement instrument by laser beam projection allows for rapid and direct determination of contact angles. The instrument may have a possibility to characterize newly developed nano-fibers. When the laser beam impinges on an edge of an interface of liquid and solid, projected beam were split across and made two straight lines on a tangent screen. From the result, it could measure the contact angle directly by reading the angle between two split beams. The purpose of this study was to prove reliability and reproducibility of the contact angle measurement instrument by laser beam projection compare to the conventional one by microscope through the comparative experiment and questionnaire. Test samples were selected by consideration of hydrophilic and hydrophobic, such as nylon 6 and polypropylene, respectively. The laser contact angle measurement has accurate, fast and convenient method to measure contact angle, and it can be a unique method to characterize nano-fibers.