An iterative modal analysis approach is developed to determine the effect of transverse open cracks on the dynamic behavior of simply supported Euler-Bernoulli beams with the moving masses. The influences of the velocities of moving masses, the distance between the moving masses and a crack have been studied on the dynamic behavior of a simply supported beam system by numerical method. The Presence of crack results In large deflection of beam. The crack section is represented by a local flexibility matrix connecting two undamaged beam segments i.e. the crack is modelled as a rotational spring. This flexibility matrix defines the relationship between the displacements and forces across the crack section and is derived by applying fundamental fracture mechanics theory. Totally, as the velocity of the moving masses and the distance between the moving masses are increased, the mid-span deflection of simply supported beam with the crack is decreased.