This paper considered the structural mechanism of transitional boundary layer by the experimental approach. In order to measure the turbulence quantity in the boundary layer, we made a wind tunnel with 400${ imes}$190${ imes}$2500 mm test section and a flat plate with well fabricated leading edge. Hot wire anemometer was used for acquiring the continuous turbulence signal which is processed by special software. The results of experiment show that the region where turbulence spot is dominant moves from near wall to overall layer and thus the anisotropy of velocity fluctuation shows so large value. Also the turbulence energy originally contained in low frequency band comes up to the high frequency band. Finally the turbulence model needs minimum two length scales to consider the pre-transition region.