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효소적 방법에 의한 방향족 알코올류 및 Monoterpene Alcohol류의 배당체 합성
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  • 효소적 방법에 의한 방향족 알코올류 및 Monoterpene Alcohol류의 배당체 합성
  • Enzymatic Synthesis of $eta$ -Glucosides of Aromatic Alcohols and Monoterpene Alcohols
저자명
김영회,김삼곤,김근수,나도영
간행물명
한국연초학회지
권/호정보
2003년|25권 1호|pp.70-79 (10 pages)
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한국연초학회
파일정보
정기간행물|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

$eta$ -Glucosidase-catalysed synthesis of glucosides with aromatic alcohols and monoterpene alcohols as accepters and cellobiose as a donor in the presence of various commercial $eta$ -glucosidases were described. $eta$ -Glucosidases from Aspergillus niger spp,. Trichoderma spp., Penicillium sup. and bitter almond have been shown to catalyze synthesis of $eta$ -glucosides of benzyl alcohol, 2-hydroxybenzyl alcohol, 4-hydroxybenzyl alcohol, 2-phenylethyl alcohol, geraniol and citronellol in the presence of cellobiose as sugar donor. Among enzyme preparations tested, each $eta$ -glucosides prepared from Aspergillus niger were isolated in the pure state by Diaion HP-20 and silica gel column chromatography. The products were identified as $eta$ -glucosyl products of benzyl alcohol, 2-hydroxyhenzyl alcohol, 4-hydroxybenzyl alcohol, 2-phenyl ethyl alcohol, geraniol and citronellol by spectrometry (UV, IR, $^1$H-NMR, $^{13}$ C-NMR) and enzymatic hydrolysis with $eta$ - glucosidase. Monoterpene alcohols with a sterically hindered hydroxyl group, such as linalool, $ell$-menthol and $alpha$-terpineol were not used as acceptors in transglycosylation reaction.