This paper represents an absolute positioning system using a light navigation path for mobile robot. The absolute positioning system is composed of the projector unit which generates a laser beam using laser diode and mobile robot with the optical detector which has some optical sensors. The projector unit is fixed over the navigating plane of mobile robot to generate the light navigation path, and the optical detector located upper part of mobile robot detects the generated laser beam from the projector. The navigation of mobile robot is controlled by the micro-processor which compares the detected present position from the detector with the previously programmed navigation path. And experimental results show that our sensor system can be used for the absolute positioning system of the mobile robot.