Freezing of turbulent water flow between two horizontal cooled parallel plates with the separated region has been investigated experimentally. The flow separation was induced by vertical plates (two-dimensional plates) situated at the inlet of the rectangular channel. The degree of flow separation was varied by employing vertical thin plates with various heights. Three kinds of the vertical plates with 8.0, 9.8 and 12.5 mm in height were utilized. The Reynolds number and cooling temperature ratio were ranged from $3.45 imes10^3 to 1.73 imes10^4$ and 7.0 to 20.0 respectively, The measurements show that the flow separation influenced remarkably on the local ice formation characteristics. The location of the first ice layer and the average heat transfer at the ice surface were found be correlated as a function of the Reynolds number, the cooling temperature ratio, and the orifice height ratio.