A mechanical or electrical stimulation to the mandibular symphysis during a maximal voluntary clenching of the teeth always produces a jaw jerk followed by a silent period (transient stops) in the masseteric EMG (electromyogram). Generally, a mechanical stimulation is followed by a single silent period, and an electrical stimulation is followed by multiple silent periods. In this paper, we propose a new algorithm for determining the duration of the masseter silent period. The decision approach in essentially based upon a segmentation algorithm consisted of variance filter, median filter and gaussian filter. The new adaptive digital notch filter using R-CLMS(reverse constrained least mean-squared) algorithm is proposed for the elimination of powerline(60Hz) noise. At the same time, we design a real time measurement system for the EMG silent period under Window base.