This study was performed to investigate the reverse effects of Jen Chung(Ren Thong, Shui Gou, GV-26) electroacupuncture after tiletamime-zolazepam administration in dogs. Seven healthy dogs ranging in weigh from 3.5 to 6.5 kg were used in this experiment. The treatment group was electrostimulated to Jen Chung(+) and Su Liao(Shan Gen, GV-25, -) for 20 minutes after 10 minutes of anesthesia. The control group I was anesthetized with tiletamine-zolazepam. The control group II was electrostimulated to nonacupuncture point for 20 minutes after 10 minutes of anesthesia. Various parameters were evaluated including the onset and recovery time of anesthesia, heart rates, body temperature, respiratory rates and electrocardiogram. The recovery time of the treatment group was shorter than that of the control group I and the control group II(p < 0.05). The treatment group had an increase in the heart rate from 20 minutes to 30 minutes after administration of tiletamime-zolazepam compared to the control group I and control group II(p < 0.05). The decrease in the body temperature was observed in all groups, but the body temperature of the treatment group was higher than the body temperature of the other groups. Respiratory rates gradually increased in all groups, but the treatment group had an increase in the respiratory rates from 20 minutes to 30 minutes after administration of tiletamime-zolazepam compared to the other groups. In the three groups, there was no specific finding on the electrocardiogram. In the result, the electroacupuncture on Jen Chung in dogs was effective for the reversed effects the tiletamine-zolazepam anesthesia 20 minutes after induction.