These studies were carried out to develop non-contact thermometer to take easily the body temperature of domestic animals instead of taking rectal temperature. For the studies, 86 cattle, 57 horses, 72 pigs, 43 goats, and 42 dogs were used and body parts as neck, flank, axilla, lateral abdomen, gluteus, inguinal region, or jugular groove were chosen for taking temperature according to different species. Two types of commercial non-contact thermometers were used to take the temperature of certain body part and at the same time the rectal temperature using digital thermo-meter was taken to compare the difference of temperature between rectum and certain body part. The difference of mean temperature in cattle between rectum and axilla and flank were 0.52 and $2.41^{circ}C$, respectively, using non-contact thermometer I, whereas $3.02^{circ}C$ between rectum and flank using thermometer II. The difference of mean temperature in horses between rectum and axilla, gluteus, and jugular groove were 0.52, 1.49, and $0.26^{circ}C$, respectively, using thermometer I, whereas 2.28 ane $0.92^{circ}C$ between rectum and gluteus or jugular groove using thermometer II. The difference of mean temperature in swine between rectum and flank, inguinal region, and neck were 1.23, 0.21, and $0.8^{circ}C$, respectively, using thermometer I, whereas 1.42, 0.711, and $1.25^{circ}C$ using thermometer II The difference of mean temperature in goats between rectum and lateral abdomen and inguinal region were 1.02 and $0.12^{circ}C$, respectively, using thermometer I, whereas 1.96 and $1.01^{circ}C$ using thermometer II. The difference of mean temperature in dogs between rectum and lateral abdomen, inguinal region, and neck were 3.26, 0.24, and $2.37^{circ}C$, respectively, using thermometer I, whereas 3.45, 0.56, and $2.61^{circ}C$ using thermometer II.