- DNA 칩 제작을 위한 로봇 시스템의 개발
- ㆍ 저자명
- 이현동,김기대,나건영,임용표
- ㆍ 간행물명
- 한국농업기계학회지
- ㆍ 권/호정보
- 2003년|28권 5호|pp.429-438 (10 pages)
- ㆍ 발행정보
- 한국농업기계학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
This study exploits the robot system which is necessary in gene study and bio-technology industry. As well, a DNA chip, which of use has been increased recently, can be manufactured with this system. The robot consists of a device spotting DNA on the silylated slide, a well plate, a bed for fixing well plates, devices of washing and drying the pin in DNA spotting .device, a distillation-water vessel, and a discharge vessel of wash water. We made the period of sticking DNA to the pin on the well plate to be 15 seconds. The spot size of DNA was set to be 0.28 mm on the average by bringing the slide into contact with pin during 1 second. If DNA is spotted in minimum space possible about 0.32mm, this system can stick about 8,100 DNA spots on the well plate with this rate. Analyzing the procedure: Movement starts, Pin washes, dries, and smears DNA on the well plate. Spotting DNA onto 12 chips took 2 minutes and 50 seconds.