This paper proposes a method to detect misfired cylinders by the exhaust pressure ascent rate. The misfire is generated by faults of electric system or faults of fuel delivery system. It is one of the abnormal combustions. Therefore, it increases the unburned hydrocarbon and the carbon monoxide and affects a bad influence to the 3-way catalyst. The misfire causes to decrease the power of the engine and increase the consumption of the fuel. Early detection and correction of the misfired cylinders can prevent these unusual phenomena. The misfired cylinders can be detected by the comparison of exhaust pressure ascent rate during each cycle. The exhaust pressure ascent rate is defined as pressure rise per time. Our experimental results showed that the proposed method is effective in the detection of the misfired cylinders on a gasoline engine regardless loads and revolutions of the engine.