Pseudomonas elodea ATCC 31461에 의해 생산되는 미생물 다당류인 gellan의 최적 조건을 확립하기 위해 flask상에서 inoculum size, 탄소원 종류 및 농도, 질소원 종류, C/N ratio등을 조사하였다. Gellan 생산을 위한 최적 inoculum size는 5%(v/v)로 나타났으며, 1.0%(w/v)의 glucose에서 2.22의 최대 specific yield 와 $0.03 g/ell$의 생산성을 얻을 수 있었다. 또한 ammonium nitrate가 없을 때 최대 생산을 얻을 수 있어, gellan 생산을 위해서 nitrogen limitation이 필요함을 알 수 있었다 Glucose와 bactopeptone을 이용하여 C/N ratio를 조사한 결과 20에서 gellan 생산이 가장 높게 나왔다.
The gellan was produced by Pseudomonas elodea under aerobic condition. In this study, the effects of inoculum size, carbon sources and concentration, nitrogen source, and C/N ratio on the cell growth and the production of gellan were evaluated. The maximum growth of P. elodea and gellan production was obtained at 5% (v/v) of inoculum size and glucose showed best results among 9 carbon sources tested. The maximum specific yield of 2.22 and productivity of $0.03 g/ell$h were obtained at 1.0% (w/v) of glucose. The maximum gellan production was obtained at medium without ammonium nitrate. This indicates that nitrogen limitation is essential for the production of gellan. The highest cell and gellan production were obtained at 20 of C/N ratio.
