A measurement of spacecraft alignment is an important process of spacecraft assembly, integration and test because it is necessary that a ground station controls the precise positions of on-orbit spacecraft by using the alignment data of attitude orbit control sensors(AOCS) on spacecraft. In addition, accuracy of spacecraft alignment requirement is about $0.1^{circ}$~$0.7^{circ}$. The spacecraft alignment is measured by autocollimation of theodolite. This paper describes the measurement principle and method of spacecraft alignment. The result shows that all of the AOCS on the spacecraft are aligned within the tolerance required through the alignment measurement.