The distortion of picosecond pulses caused by dispersion as it propagates along microstripline and CPW(Coplanar waveguide) is investigated and measured using time domain measurement. Especially, this study presents that the distortion of these lines is analyzed and compared considering the relation between the dispersion effect and cut-off frequency(f$\_$TE/) of fundamental TE mode for the substrate of line. To verify theoretical results, several samples of 50 $Omega$ lines are fabricated on the two kind of substrates with the different values of f$\_$TE/. The propagation characteristics of picosecond Gaussian pulse on thess lines are simply measured using VNA(Vector Network Analyzer) with the time-domain analysis function.