This study aims to understand the correlation between system of exhibition contents and structure of exhibition space in nature and science museums. So, in this paper it deals with 10 examples of overseas nature and science museums as analytical objects, using Space Syntax as an analytical tool. In conclusion, the system of exhibition contents of nature & science museums can be divided into field and theme, and subdivided into total 6 systems according to categorization and integration. The system of exhibition contents by field is generally characterized by unconstricted access and system of exhibition contents by theme is generally marked by high visual openness. And the system of exhibition content according to categorization is characterized by strong concentration on exhibitory convex and its wide distribution.