This study develops an efficient heuristic which is based on genetic approach for AGVs flow path layout problem. The suggested solution approach uses a algorithm to replace two 0-1 integer programming models and a branch-and-bound search algorithm. Genetic algorithms are a class of heuristic and optimization techniques that imitate the natural selection and evolutionary process. The solution is to determine the flow direction of line in network AGVs. The encoding of the solutions into binary strings is presented, as well as the genetic operators used by the algorithm. Genetic algorithm procedure is suggested, and a simple illustrative example is shown to explain the procedure.