Many Korean entomologists have attempted to colonize Anopheles sinensis Korean strain, a primary malaria vector in Korea, previous efforts, however, were unsuccessful. Only a study that a successful colonization of An. sinensis Korean strain over 5 generations was recently reported. Recently we obtained a self­mating colony from this mosquito strain. The original colony was established from progenies obtained from females collected from So­Rae District, Incheon, Republic of Korea (ROK), and was maintained for 23 successive generations. While the adult females of first five generations were fed on humans as a source of blood, those of the subsequent 18 generations were fed on white rats as a source of blood. A self­mating colony was initiated from the $9^{th}$ generation by rearing 200 and 300 newly emerged females and males, respectively, in a $30{ imes}30{ imes}30;cm$ cage. The insemination rates increased from approximately 40％ to 81％ by the $20^{th}$ generation. Comparisons of biological aspects between artificial mating and self­mating colonies are reported.